Genome of a thermophilic bacterium Geobacillus sp. TFV3 from Deception Island, Antarctica
Genome of a thermophilic bacterium Geobacillus sp. TFV3 from Deception Island, Antarctica
at : Jan 14, 2020 09:39:15  (view:16)

Ching, X.J.1, Teoh, C.P.1, Lee, D.J.H. 1, González, M. A. 2, Najimudin, N. 3, Cheah, Y.K.4, Lavin, P.5 and Wong, C.M.V.L.1,6*

1Biotechnology Research Institute, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia;

2Instituto Antártico Chileno, Plaza Muñoz Gamero 1055, Punta Arenas, Chile;

3 School of Biological Science, Universiti Sains Malaysia, Persiaran Bukit Jambul

11900 Bayan Lepas, Penang, Malaysia;

4Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia;

5Laboratorio de Complejidad Microbiana y Ecología Funcional, Instituto Antofagasta, Universidad de Antofagasta, Chile;

6National Antarctic Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia

Abstract: Thermophilic microorganisms have always been an important part of the ecosystem, particularly in a hot environment, as they play a key role in nutrient recycling at high temperatures where most microorganisms can not cope. While most of the thermophiles are archaea, thermophiles can also be found among some species of bacteria. These bacteria are very useful in the fundamental study of heat adaptation, and they are also important as potential sources of thermostable enzymes and metabolites. Recently, we have isolated a Gram-positive thermophilic bacterium, Geobacillus sp. TFV3 from a volcanic soil sample from Deception Island, Antarctica. This project was undertaken to analyze the genes of this thermophilic Antarctic bacterium, and to determine the presence of thermal-stress adaptation proteins in its genome. The genome of Geobacillus sp. TFV3 was first purified, sequenced, assembled, and annotated. The complete genome was then found to harbor genes encoding for useful thermal-stress adaptation proteins. The majority of these proteins were categorized under the family of molecular chaperone and heat shock protein. This genomic information could eventually provide insights on how the bacterium adapts itself towards high growth temperatures.

Keywords: Temperature, 16S rDNA, genomes, Geobacillus, Deception Island

*Corresponding Author:

Prof. Dr. Clemente Michael Wong Vui Ling